Effect of Agitation Rate on Growth Yield and Virulence of Bordetella pertussis Strain 509 in Lab Scale Cultivation

Inhaltsverzeichnis (Table of Contents)

• Abstract
• Keywords
• Introduction
• Material and Methods
  • Preparation of Media
  • Opacity Test
  • Sterility Test
  • Effect of Agitation
  • Mouse Weight Gain Test
  • LPF Activity
  • Filamentous Haemagglutinin (FHA) Adherence Activity towards Vero & CHO Cell Line
• Results and Discussion
• Conclusion
• Reference

Zielsetzung und Themenschwerpunkte (Objectives and Key Themes)

This research article investigates the impact of agitation rate on the growth yield and virulence of Bordetella pertussis strain 509 during pilot scale cultivation for whooping cough vaccine preparation. The study aims to determine the optimal agitation rate for efficient bacterial growth while maintaining the desired virulence properties and minimizing toxicity.

• Effect of agitation rate on growth yield and virulence of Bordetella pertussis
• Shear stress on bacterial cells and its impact on virulence factors
• Assessment of toxicity through mouse weight gain and lymphocytosis promoting factor tests
• Evaluation of the adherence activity of filamentous hemagglutinin (FHA) to Vero and CHO cell lines
• Correlation between agitation rate, cell lysis, and vaccine quality

Zusammenfassung der Kapitel (Chapter Summaries)

The introduction provides background information on Bordetella pertussis, the causative agent of whooping cough, and the importance of pertussis vaccines. It also outlines the various toxins and virulence factors produced by the bacterium.

The materials and methods section details the experimental procedures employed, including the preparation of culture media, the opacity test, the sterility test, and the methods used to assess the effect of agitation on bacterial growth, virulence, and adherence. The mouse weight gain test, lymphocytosis promoting factor (LPF) activity, and FHA adherence assays are also explained.

The results and discussion section presents the findings from the experiments, analyzing the impact of agitation rate on bacterial growth, toxicity, and FHA adherence. The data is presented in tables and figures, and the significance of the findings is discussed in relation to previous research.

Schlüsselwörter (Keywords)

The main keywords and focus topics of the text are: agitation rate, Bordetella pertussis, endotoxin, toxicity, virulence, FHA, cell lysis, vaccine production, whooping cough, pertussis, growth yield, LPF activity, Vero cells, CHO cells, bioreactor, shear stress.