The Lymphatic Fluid and T-Cell Receptor Vβ expression in Peripheral Blood Mononuclear Cells (PBMCs) of patients with Wuchereria Bancrofti Infection

Inhaltsverzeichnis (Table of Contents)

• Abstract
• Acknowledgement

Zielsetzung und Themenschwerpunkte (Objectives and Key Themes)

This thesis aims to investigate the immunopathological mechanisms underlying chronic pathology (CP) in lymphatic filariasis (LF) caused by Wuchereria bancrofti infection. The study focuses on identifying parasite-specific antigens and antibodies in lymphatic fluid from CP patients and comparing them to serum samples from the same patients. It also explores T-cell receptor (TCR) Vβ gene expression in peripheral blood mononuclear cells (PBMCs) stimulated with parasite antigens.

• Identification of parasite-specific antigens and antibodies in lymphatic fluid and serum of CP patients.
• Analysis of TCR Vβ gene expression in PBMCs of CP patients in response to parasite antigens.
• Investigation of the effect of lymphatic fluid on bacterial growth.
• Comparison of immune responses in CP patients, normal healthy individuals (EN), and microfilaria carriers (MF).
• Understanding the role of immune reactivity in the development of elephantiasis.

Zusammenfassung der Kapitel (Chapter Summaries)

The abstract provides an overview of lymphatic filariasis, the study's objectives, and a summary of the key findings. The acknowledgement section expresses gratitude to individuals and institutions that supported the research.

Schlüsselwörter (Keywords)

Lymphatic filariasis, Wuchereria bancrofti, chronic pathology, lymphatic fluid, serum, antigens, antibodies, T-cell receptor (TCR), Vβ gene expression, PBMCs, immunopathology, elephantiasis, bacterial infections, SDS-PAGE, Western blot, RT-PCR.