Identification of Species Specific DNA Marker as Barcode Sequence from Greater Indian One-Horned Rhinoceros (Rhinoceros unicornis) from North East India

Inhaltsverzeichnis (Table of Contents)

• CHAPTER-1 Introduction
• CHAPTER- 2 Review of literature
• CHAPTER- 3 Materials and methods
• CHAPTER -4 Results
• CHAPTER - 5 Discussion
• REFERENCES

Zielsetzung und Themenschwerpunkte (Objectives and Key Themes)

This project report aims to identify a species-specific DNA marker, serving as a barcode sequence, for the Greater Indian One-Horned Rhinoceros (Rhinoceros unicornis) found in Northeast India. The research investigates the use of DNA barcoding techniques for species identification and conservation efforts.

• Identification of a species-specific DNA marker
• Application of DNA barcoding for species identification
• Conservation implications of DNA barcoding techniques
• Genetic diversity of the Greater Indian One-Horned Rhinoceros
• Role of molecular techniques in wildlife conservation

Zusammenfassung der Kapitel (Chapter Summaries)

CHAPTER-1 Introduction: This chapter introduces the significance of DNA barcoding for species identification and conservation, focusing on the challenges faced by the Greater Indian One-Horned Rhinoceros. It discusses the importance of molecular tools in wildlife conservation and the potential of DNA barcoding for monitoring rhinoceros populations. CHAPTER- 2 Review of literature: This chapter presents a comprehensive review of existing literature on DNA barcoding, focusing on its applications in wildlife conservation and species identification. It explores various DNA barcode regions and their effectiveness in differentiating rhinoceros species. CHAPTER- 3 Materials and methods: This chapter outlines the materials and methodologies used in the project. It details the sampling procedures, DNA extraction techniques, PCR amplification, sequencing protocols, and data analysis methods employed. CHAPTER -4 Results: This chapter presents the findings of the study, including the identification of potential DNA barcode regions, their sequence variations, and the development of a species-specific DNA marker for the Greater Indian One-Horned Rhinoceros.

Schlüsselwörter (Keywords)

DNA barcoding, species identification, conservation, Greater Indian One-Horned Rhinoceros (Rhinoceros unicornis), genetic diversity, mitochondrial DNA, cytochrome oxidase subunit I (COI), PCR, sequencing, data analysis.