Micropropagation of Rosa centifolia

Inhaltsverzeichnis (Table of Contents)

• INTRODUCTION
• REVIEW OF LITERATURE
  • Other ornamental plants
  • Fruit plants
• MATERIALS AND METHODS
  • Explant collection
  • Preparation of medium
  • Sterilization of explant and culture
  • Browning
  • Media used
    • Media for browning
    • Media for shooting
    • Media for rooting
  • Cultural conditions
  • Data collection
    • Shoot multiplication
      • Number of days to sprout
      • Number of laterals
      • Shoots length (cm)
      • Number of leaves/plant
    • Root initiation
      • Number of days to emerge roots
      • Number of roots
      • Root length (cm)
    • Browning
• RESULTS AND DISCUSSION
  • Number of days to initiate shoots
  • Number of laterals
  • Number of leaves/explant
  • Shoot length
  • Number of days to emerge roots
  • Number of roots
  • Root length
  • Acclimatization
  • Browning control
• SUMMARY
  • Conclusion
• LITARATURE CITED

Zielsetzung und Themenschwerpunkte (Objectives and Key Themes)

This thesis explores the micropropagation of Rosa centifolia, aiming to optimize in vitro techniques for efficient multiplication and root initiation. The research investigates the effects of various growth regulators and cultural conditions on the growth and development of the plant.

• The impact of different growth regulators on shoot multiplication and root development
• Optimization of media composition for shoot and root induction
• Evaluation of the influence of various cultural conditions on plant growth and development
• Acclimatization of micropropagated plants to ex vitro conditions
• Control of browning, a common problem in micropropagation

Zusammenfassung der Kapitel (Chapter Summaries)

The initial chapters provide a comprehensive introduction to Rosa centifolia and its significance in horticulture. The review of literature delves into established micropropagation techniques and their applications in ornamental plants, including Rosa centifolia. Chapter 3 outlines the materials and methods used in the study, focusing on the procedures for explant collection, medium preparation, sterilization, browning control, and data collection. The core results and discussions are presented in Chapter 4, analyzing the impact of various growth regulators on shoot emergence, lateral shoot formation, leaf production, shoot length, root emergence, root number, root length, and acclimatization. The chapter also explores browning control strategies. The summary in Chapter 5 provides a comprehensive overview of the findings, highlighting the conclusions drawn from the research.

Schlüsselwörter (Keywords)

The study focuses on the micropropagation of Rosa centifolia, exploring key topics such as in vitro culture, shoot multiplication, root induction, growth regulators, cultural conditions, acclimatization, and browning control.