Bachelorarbeit, 2013
86 Seiten, Note: M.Sc.
1. INTRODUCTION
2. METHANOL EXTRACT AND SILVER NANOPARTICLES
3. ACETONE EXTRACT AND SILVER NANOPARTICLES
4. WATER EXTRACT AND SILVER NANOPARTICLES
5. LITERATURE CITED
The primary research objective of this work is to investigate a green synthesis methodology for producing silver nanoparticles (AgNPs) using the bark extract of the medicinal plant Acacia auriculiformis, followed by a comprehensive comparative evaluation of their antimicrobial and antioxidant potential against crude plant extracts.
INTRODUCTION
Research and analysis of nanoparticles (NPs) synthesis and their biological activities has been expanded significantly in the recent years. The agents used for nanoparticles (NPs) synthesis are of organic (mainly carbon) and inorganic (metal ions like silver and gold) origin (Singh et al., 2010). Among these, silver (Ag) is the most preferred NPs synthesis agent due to its reported use in medical field as best topical bactericides from ancient times (Lavanya et al., 2013). The stable silver nanoparticles had been synthesized by using soluble starch as both the reducing and stabilizing agents (Shrivastava et al., 2012). So the concern of scientific community shifted towards ecofriendly, natural and cheaper method of NPs synthesis by using microorganisms and plant extracts (Mohanpuria et al., 2008). The use of plant materials for silver nanoparticles (AgNPs) is most popular due to its potential biological activities, easy availability and faster rate of synthesis there by cutting the cost of NP's synthesis (Huang et al., 2007 and Salam et al., 2012).
Nanoparticles could be synthesized by various approaches like photochemical reactions in reverse micelles (Taleb et al., 1997), thermal decomposition (Esumi et al., 1990), sonochemical (Zhu et al., 2000) and microwave assisted process (Santosh et al., 2002 and Prasher et al., 2009). Nanocrystalline silver particles have found tremendous applications in the field of high sensitivity biomolecular detection and diagnostics (Schultz et al., 2000), antimicrobials and therapeutics (Rai and Yadav., 2009 and Elechiguerra et al., 2005) and micro-electronics (Gittins et al., 2000).
1. INTRODUCTION: Provides an overview of nanoparticle synthesis methods, emphasizing green synthesis using plant extracts, and introduces the medicinal properties of Acacia auriculiformis.
2. METHANOL EXTRACT AND SILVER NANOPARTICLES: Details the synthesis and characterization of silver nanoparticles using methanol extracts of A. auriculiformis and analyzes their antimicrobial and antioxidant effectiveness.
3. ACETONE EXTRACT AND SILVER NANOPARTICLES: Examines the experimental results of using acetone-based bark extracts to synthesize silver nanoparticles and compares their bioactivity to the crude extract.
4. WATER EXTRACT AND SILVER NANOPARTICLES: Focuses on the green synthesis of nanoparticles using water-based extraction and evaluates their specific performance in antimicrobial and antioxidant assays.
5. LITERATURE CITED: A comprehensive collection of academic references and research studies utilized throughout the thesis.
Silver nanoparticles, AgNPs, green synthesis, Acacia auriculiformis, antimicrobial activity, antioxidant capacity, plant extract, methanol extract, acetone extract, water extract, biosynthesis, UV-Visible spectroscopy, FTIR, nanotechnology.
The research focuses on the green synthesis of silver nanoparticles (AgNPs) using bark extracts of the plant Acacia auriculiformis as a natural and eco-friendly reducing agent.
The study covers nanotechnology, phytochemistry, and microbiology, specifically focusing on the bioactivity of synthesized nanoparticles and their potential therapeutic applications.
The objective is to synthesize AgNPs using three different solvents (methanol, acetone, water), characterize them using spectroscopic techniques, and compare their antimicrobial and antioxidant potential against the crude extracts.
The study uses maceration extraction, green synthesis through chemical reduction, and characterization via UV-Vis, FTIR, and Photoluminescence spectroscopy. Biological activity is assessed using agar well diffusion and antioxidant biochemical assays.
The main chapters detail the experimental protocols and results for each solvent-specific extract, including the physical characterization of the produced AgNPs and their measured bio-efficacy.
Key terms include green synthesis, AgNPs, Acacia auriculiformis, antimicrobial activity, antioxidant capacity, and biosynthesized nanoparticles.
The results suggest that solvent polarity plays a critical role, as higher polarity often correlates with the extraction efficiency of polyphenols and flavonoids, which contribute significantly to the antioxidant scavenging potential of the synthesized AgNPs.
The bark serves as a rich source of polyphenols and tannins, acting as both a reducing and a capping agent in the green synthesis process, which facilitates the stable formation of silver nanoparticles.
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