Antioxidant Activity of Selected Wild Orchids of Nepal

Inhaltsverzeichnis (Table of Contents)

• ABSTRACT
• INTRODUCTION
• REVIEW OF LITERATURE
• Oxidative Stress
• Antioxidants and their Sources
• Mechanism of Antioxidant Action
• Antioxidant Activity of Orchids
• MATERIAL AND METHODS
• Plant Materials
• Chemicals and Reagents
• Preparation of Extracts
• Phytochemical Analysis
• Total Flavonoids Content (TFC)
• Total Polyphenolics Content (TPC)
• Antioxidant Activity
• DPPH Radical Scavenging Assay
• Statistical Analysis
• RESULTS AND DISCUSSION
• Phytochemical Screening
• Total Flavonoids Content (TFC)
• Total Polyphenolics Content (TPC)
• Antioxidant Activity of Extracts
• DPPH Radical Scavenging Activity
• Correlation Studies
• CONCLUSION
• REFERENCES

Zielsetzung und Themenschwerpunkte (Objectives and Key Themes)

This dissertation aims to investigate the antioxidant activity of nine wild orchid species found in Nepal. The study explores the relationship between the total flavonoids and polyphenolics content in these orchids and their antioxidant properties.

• Investigate the antioxidant activity of selected wild orchids of Nepal.
• Determine the total flavonoids and polyphenolics content in the orchid extracts.
• Assess the correlation between antioxidant activity and the levels of flavonoids and polyphenolics.
• Compare the antioxidant activity of the orchid extracts to established reference compounds.
• Explore the potential of these orchids as sources of natural antioxidants.

Zusammenfassung der Kapitel (Chapter Summaries)

The introduction provides an overview of oxidative stress, antioxidants, and their sources. The review of literature delves into the mechanism of antioxidant action and explores previous research on the antioxidant activity of orchids. The material and methods section outlines the experimental procedures, including plant collection, extraction methods, and analysis techniques. Results and discussion present the findings of the study, focusing on the phytochemical analysis of the orchid extracts, their antioxidant activity, and the correlation between these factors. The conclusion summarizes the major findings and discusses the potential applications of the study's results.

Schlüsselwörter (Keywords)

The key focus areas of this dissertation are antioxidant activity, wild orchids, flavonoids, polyphenolics, DPPH assay, Nepal, and ethanolic extracts.