Masterarbeit, 2013
79 Seiten, Note: A
This dissertation aims to isolate quercetin from Allium cepa (onion) and to evaluate the total phenolic and flavonoid content, as well as the antioxidant activities of four common medicinal plants: Ficus benghalensis, Elaeocarpus sphaericus, Ipomea carnea, and Azaratum conyzoides.
Chapter 1 provides a comprehensive overview of the research topic, including the background of quercetin and its health benefits, a review of relevant literature, and the specific objectives and significance of the study. Chapter 2 outlines the materials and methods used in the study, including the collection and preparation of plant materials, the isolation of quercetin from onion, the quantification of total phenolic and flavonoid content, and the evaluation of antioxidant activities using various assays. Chapter 3 presents the results and discussion, focusing on the characterization of isolated quercetin, the determination of phenolic and flavonoid content, and the analysis of antioxidant activities. The chapter also provides a detailed interpretation of the results in relation to previous research and the potential applications of the studied plants.
Quercetin, Allium cepa, Ficus benghalensis, Elaeocarpus sphaericus, Ipomea carnea, Azeratum conyzoides, medicinal plants, phenolic compounds, flavonoids, antioxidant activity, DPPH radical scavenging activity, reducing power assay.
Quercetin was isolated from the outer scale of onions (Allium cepa) using a methanol extract, followed by repeated Sephadex LH-20 column chromatography.
The study screened Ficus benghalensis, Elaeocarpus sphaericus, Ipomea carnea, and Azeratum conyzoides from the Kathmandu region of Nepal.
The study found a strong linear correlation (R² = 0.931), indicating that phenolic compounds are the primary contributors to the antioxidant properties of these plants.
The 70% aqueous acetone extract of Elaeocarpus sphaericus contained the highest amount of phenolics (298.769 mg GAE/g).
Antioxidant activity was evaluated using the DPPH radical scavenging assay and the reducing power assay.
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