The influence of hypoxia on GATA-1 and Epo expression levels in developing zebrafish

Inhaltsverzeichnis (Table of Contents)

• Abstract
• Chapter 1
  • Introduction
  • Zebrafish hematopoiesis
  • GATA-1 and Co-factors
    • The role of GATA-1
    • GATA-1 related factors
  • Epo and EpoR
  • Oxygen dependent development
  • Intention of this study
• Chapter 2
  • Material and Methods
  • Animals
  • Imaging system
  • Epo mRNA
  • Statistics
• Chapter 3
  • Results
  • General aspects
  • Total cell number
  • Manually counted fluorescent cells
  • GATA-1 expression
  • Epo expression
• Chapter 4
  • Discussion
  • General aspects and observations
  • Pros and cons of DsRed
  • Epo and GATA-1
  • In vivo imaging

Zielsetzung und Themenschwerpunkte (Objectives and Key Themes)

This master's thesis investigates the influence of hypoxia on the expression levels of GATA-1 and erythropoietin (Epo) in developing zebrafish. The study aims to understand the relationship between these genes and the development of red blood cells, as well as the role of oxygen availability in this process.

• Zebrafish hematopoiesis and its role in development
• The function of GATA-1 and its co-factors in erythroid cell development
• The role of Epo and its receptor (EpoR) in erythropoiesis
• The influence of hypoxia on the expression of GATA-1 and Epo
• In vivo imaging techniques for studying gene expression and cell development

Zusammenfassung der Kapitel (Chapter Summaries)

• Chapter 1: Introduction This chapter provides an overview of zebrafish hematopoiesis, the function of GATA-1 and Epo in red blood cell development, and the role of oxygen in this process. It also introduces the study's objectives and methodology.
• Chapter 2: Material and Methods This chapter describes the experimental procedures used in the study, including the zebrafish model, imaging system, and techniques for measuring gene expression and cell counts.
• Chapter 3: Results This chapter presents the findings of the study, including the analysis of blood cell concentration, GATA-1 expression, and Epo expression levels in developing zebrafish larvae.
• Chapter 4: Discussion This chapter interprets the results of the study and discusses their implications for understanding the role of GATA-1, Epo, and oxygen in the development of red blood cells in zebrafish.

Schlüsselwörter (Keywords)

The primary focus of this thesis is on the influence of hypoxia on the expression levels of GATA-1 and Epo in developing zebrafish. The research explores key concepts such as zebrafish hematopoiesis, erythropoiesis, transcription factors, gene expression, in vivo imaging, and the role of oxygen in developmental processes.