Antibacterial activity of four plant species used in traditional medicine practice of South Omo Zone, Southern Ethiopia

Inhaltsverzeichnis (Table of Contents)

• Acknowledgments
• Abbreviations and Acronyms
• Table of Contents
• List of Tables
• Abstract
• CHAPTER ONE
  • INTRODUCTION
  • 1.1. Background
  • 1.2. Statement of the Problem
  • 1.3. Significance of the Study
• CHAPTER TWO
  • REVIEW OF LITERATURES
  • 2.1. Antimicrobials and Antimicrobial Resistance Infections
  • 2.2. Plant Based Antimicrobial Studies in Ethiopia
  • 2.3. Ethnobotanical Data of Medicinal Plants in Omo Valley
  • 2.4. Review on Plants Selected for the Experiment
    • 2.4.1. Aloe pirottae Berger
    • 2.4.2. Grewia schweinfurthii Burret
    • 2.4.3. Kosteletzkya begoniifolia (Ulber) Ulber
    • 2.4.4. Uvaria leptocladon Oliv
• CHAPTER THREE
  • OBJECTIVES
  • 3.1. General Objective
  • 3.2. Specific Objectives
• CHAPTER FOUR
  • MATERIALS AND METHODS
  • 4.1. Description of the Study Area
  • 4.2. Study Design and Period
  • 4.3. Selection of Medicinal Plants
  • 4.4. Plant Material Collection and Transportation
  • 4.5. Taxonomic Identification of Plants
  • 4.6. Processing of Medicinal Plants
  • 4.7. Preparation of Plant Extract
  • 4.8. Test Microorganisms
  • 4.9. Antibacterial Assay
    • 4.9.1. Primary Antibacterial Screening Against ATCC strains
    • 4.9.2. Extended Antibacterial Screening Against MDR Clinical Isolates
  • 4.10. Determining Minimum Inhibitory Concentration
  • 4.11. Quality Control
  • 4.12. Statistical Analysis
  • 4.13. Ethical Consideration
  • 4.14. Oprational Definitions
• CHAPTER FIVE
  • RESULTS
  • 5.1. Overall Primary Antibacterial Screening Against ATCC Bacteria
    • 5.1.1. Primary Antibacterial Activity of A. pirottae crude extracts against ATCC
    • 5.1.2. Primary Antibacterial Activity of G. schweinfurthii crude extracts against ATCC
    • 5.1.3. Primary Antibacterial Activity of K. begoniifolia crude extracts against ATCC
    • 5.1.4. Primary Antibacterial Activity of U. leptocladon crude extracts against ATCC
  • 5.2. Extended Antibacterial Screening Against MDR Clinical Isolates
    • 5.2.1. Extended Antibacterial Activity of K. begoniifolia Against MDR Clinical Isolates
    • 5.2.2. Extended Antibacterial Activity of U. leptocladon Against MDR Clinical Isolates
  • 5.3. Determination of MIC of U. leptocladon extract against ATCC strains
  • 5.4. Determination of MIC of U. leptocladon extract against MDR clinical isolates
• CHAPTER SIX
  • DISCUSSIONS

Zielsetzung und Themenschwerpunkte (Objectives and Key Themes)

This research thesis aims to investigate the antibacterial activity of four plant species commonly used in traditional medicine practices in the South Omo Zone of Southern Ethiopia. The study explores the potential of these plants to combat antimicrobial resistance, a growing global health concern.

• Antibacterial activity of plant species used in traditional medicine
• Evaluation of the effectiveness of plant extracts against common bacterial strains
• Assessment of the potential of these plants as a source of novel antibacterial agents
• Contribution to the understanding of traditional medicine practices and their relevance to modern healthcare
• Addressing the challenge of antimicrobial resistance through the investigation of natural remedies

Zusammenfassung der Kapitel (Chapter Summaries)

The thesis begins by outlining the background of the study, highlighting the significance of antimicrobial resistance and the potential of plant-based solutions. Chapter Two provides a comprehensive review of existing literature related to antimicrobial resistance, plant-based antimicrobial studies in Ethiopia, and ethnobotanical data on medicinal plants found in the Omo Valley. This chapter also focuses on the four specific plants selected for the study, reviewing their traditional uses and known properties. Chapter Three outlines the general and specific objectives of the research, which aim to determine the antibacterial activity of the selected plants against specific bacterial strains, both from standard cultures and clinical isolates. Chapter Four details the materials and methods used in the study, including the description of the study area, the selection and collection of plant materials, the preparation of plant extracts, the bacterial strains used, and the methodologies employed for the antibacterial assays. Chapter Five presents the results of the study, including the primary antibacterial screening of all four plant species against standard bacterial strains and the extended screening of two promising species against multidrug resistant (MDR) clinical isolates. This chapter also includes the determination of the minimum inhibitory concentration (MIC) of the most effective plant extract against both standard and MDR bacterial strains. Chapter Six provides an in-depth discussion of the results, analyzing the findings in the context of existing literature and highlighting the significance of the study's conclusions.

Schlüsselwörter (Keywords)

The research focuses on traditional medicine, plant-based antimicrobials, antimicrobial resistance, South Omo Zone, Ethiopia, antibacterial activity, minimum inhibitory concentration, ethnobotany, medicinal plants, and clinical isolates.